Cell division mechanics:
*Equal contribution
Neahring L, He Y, Cho NH, Liu G, Fernandes H, Rux CJ, Nakos K, Subramanian R, Upadhyayula S, Yildiz A, Dumont S. Torques within and outside the human spindle balance twist at anaphase. Journal of Cell Biology (in revision, 2024). bioRxiv
Chong MK, Rosas Salvans M, Tran V, Dumont S. Chromosome size-dependent polar ejection force impairs mammalian mitotic error correction. Journal of Cell Biology (in press, 2024). bioRxiv
Sutanto R*, Neahring L*, Serra Marques A, Kilinc S, Goga A, Dumont S. The oncogene cyclin D1 promotes bipolar spindle integrity under compressive force. Plos One https://doi.org/10.1371/journal.pone.0296779 (2024). [PDF]
Richter, M. Probing cytoskeletal remodelling by cutting and marking filaments. Nature Reviews Molecular Cell Biology, doi: 10.1038/s41580-023-00687-8 (2023). [PDF]
Bakshi A, Echegaray Iturra F, Alamban A, Rosas-Salvans M, Dumont S, Aydogan MG. Cytoplasmic divisions without nuclei. Cell 186, 694-4709 (2023). [PDF] [bioRxiv]
Richter R, Neahring L, Tao J, Sutanto R, Cho NH, Dumont S. Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindle. eLife 12, e85208 (2023). [PDF] [bioRxiv]
Valdez VA*, Neahring L*, Petry S*, Dumont S*. Spindle Assembly: From Molecular Parts to Robust Machine. Nature Reviews Molecular Cell Biology, doi: 10.1038/s41580-023-00584-0 (2023). [PDF]
Suresh P*, Galstyan V*, Phillips R+, Dumont S+. Modeling and mechanical perturbations reveal how spatially regulated anchorage gives rise to spatially distinct mechanics across the mammalian spindle. eLife 11:e79558 (2022). [PDF] [bioRxiv] [arXiv]
Kai FB, Ou G, Tourdot RW, Stashko C, Gaietta G, Swift MF, Volkmann N, Long AF, Han Y, Huang HH, Northey JJ, Leidal AM, Viasnoff V, Bryant DM, Guo W, Wiita AP, Guo M, Dumont S, Hanein D, Radhakrishnan R, Weaver VM. ECM dimensionality tunes actin tension to modulate the endoplasmic reticulum and spheroid phenotype. EMBO Journal 41, e109205 (2022). [PDF]
Rosas-Salvans M, Sutanto R, Suresh P, Dumont S. The Astrin-SKAP Complex Reduces Friction at the Kinetochore-Microtubule Interface. Current Biology 32, 1-11 (2022). [PDF] [bioRxiv]
Dispatch by Cédric Castrogiovanni and PatrickMeraldi
Neahring L, Cho N, Dumont S. Opposing motors provide mechanical and functional robustness in the human spindle. Developmental Cell 56, 1-13 (2021) [PDF]. [bioRxiv]
Serra-Marques A, Houtekamer R, Hintzen D, Canty JT, Yildiz A, Dumont S. The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics. Journal of Cell Biology 219, e202004202 (2020). [PDF] [bioRxiv]
PreLight by Agathe Chaigne and Gautam Day.
Long AF, Suresh P, Dumont S. Individual kinetochore-fibers locally dissipate force to maintain robust mammalian spindle structure. Journal of Cell Biology 219: e201911090 (2020). [PDF] [bioRxiv]
JCB Editors’ Choice 2021
Suresh P, Long AF, Dumont S. Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes. eLife 9: e53807 (2020). [PDF] [bioRxiv]
Rohrberg J, Van de Mark D, Amouzgar M, Lee JV, Taileb M, Corella A, Kilinc S, Williams J, Jokisch ML, Camarda R, Balakrishnan S, Shankar R, Zhou A, Chang AN, Chen B, Rugo HS, Dumont S, Goga A. Cell Reports 30, 3368–3382 (2020). [PDF]
Kuhn J, Dumont S. Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner. Journal of Cell Biology 218, 3583-3596 (2019). [PDF] [bioRxiv]
Long AF, Kuhn J, Dumont S. The mammalian kinetochore-microtubule interface: robust mechanics and computation with many microtubules. Current Opinion in Cell Biology 60, 60–67 (2019). [PDF]
Hueschen CL, Galstyan V, Amouzgar M, Phillips R, Dumont S. Microtubule end-clustering maintains a steady-state spindle shape. Current Biology 29, 700-708 (2019). [PDF]
Shah KN, Bhatt R, Rotow J, Rohrberg J, Olivas V, Wang VE, Hemmati G, Martins MM, Maynard A, Kuhn J, Galeas J, Donnella HJ, Kaushik S, Ku A, Dumont S, Krings G, Haringsma HJ, Robillard L, Simmons AD, Harding TC, McCormick F, Goga A, Blakely CM, Bivona TG, Bandyopadhyay S. Aurora kinase A drives the evolution of resistance to third-generation EGFR inhibitors in lung cancer. Nature Medicine 25, 111-118 (2019). [PDF]
Neahring L, Dumont S. Chromosome Segregation Fidelity Integrates Information across Scales. Developmental Cell 47, 525-526 (2018). [PDF]
Elting MW*, Suresh P*, Dumont S. The spindle: Integrating architecture and mechanics across scales. Trends in Cell Biology 28, 896-910 (2018). [PDF]
Serra-Marques A, Dumont S. Cell Division: Shining light on spindle positioning. eLife, 7:e38748 (2018). [PDF]
Hueschen CL, Kenny SJ, Xu K, Dumont S. NuMA recruits dynein activity to microtubule minus-ends at mitosis. eLife, 6:e29328 (2017). [PDF] [bioRxiv]
Elting MW, Prakash M, Udy DB, Dumont S. Mapping load-bearing in the mammalian spindle reveals local kinetochore-fiber anchorage that provides mechanical isolation and redundancy. Current Biology 27, 2112-2122 (2017). [PDF] [bioRxiv]
Guild J, Ginzberg MB, Hueschen CL, Mitchison TJ, Dumont S. Increased lateral microtubule contact at the cell cortex is sufficient to drive mammalian spindle elongation. Molecular Biology of the Cell 28, 1975-1983 (2017). [PDF]
Long AF, Udy DB, Dumont S. Hec1 tail phosphorylation differentially regulates mammalian kinetochore coupling to polymerizing and depolymerizing microtubules. Current Biology 27, 1692-1699 (2017). [PDF] [bioRxiv]
Kuhn J, Dumont S. Spindle assembly checkpoint satisfaction occurs via end-on but not lateral attachments under tension. Journal of Cell Biology 216, 1533-1542 (2017). [PDF] [bioRxiv]
Karg T, Elting MW, Vicars H, Dumont S, Sullivan W. The chromokinesin Klp3a and microtubules facilitate acentric chromosome segregation. Journal of Cell Biology 216, 1597-1608 (2017). [PDF]
Guild J*, Haque A*, Gheibi P, Gao Y, Son KJ, Foster E, Dumont S, Revzin A. Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF. Stem Cells: 10.1002/stem.2324 (2016). [PDF] (cover of next issue)
Hueschen CL, Long AF, Dumont S. Kinesin-5: A Team Is Just the Sum of Its Parts. Developmental Cell 24, 609-610 (2015). [PDF]
Udy DB, Voorhies M, Chan PP, Lowe TM, Dumont S. Draft De Novo Transcriptome of the Rat Kangaroo Potorous tridactylus as a Tool for Cell Biology. PLoS One 10: e0134738 (2015). [PDF]
Data: Publicly available on our lab website (data browsers) and on the PlosOne website (raw data).
Dumont S, Prakash M. Emergent mechanics of biological structures. Molecular Biology of the Cell 25, 3461-3465 (2014). [PDF]
Elting MW*, Hueschen CL*, Udy DB, Dumont S. Force on spindle microtubule minus-ends moves chromosomes. Journal of Cell Biology 206, 245-256 (2014). [PDF]
Commentaries: Biobytes podcast, F1000
Kuhn J, Dumont S. Imaging and physically probing kinetochores in live dividing cells. Methods in Cell Biology 123, 467-87 (2014). [LINK]
Dumont S. Spindle size: Small droplets and a big step forward. Current Biology 24, R116-118 (2014). [PDF]
Dumont S, Salmon ED, Mitchison TJ. Deformations within moving kinetochores reveal different sites of active and passive force generation. Science 337, 355-358 (2012). [PDF]
Commentaries: HMS, F1000
Dumont S, Mitchison TJ. Mechanical Forces in Mitosis. Book chapter in: Edward H. Egelman, editor: Comprehensive Biophysics, Vol 4, Molecular Motors and Motility, Yale E. Goldman, E. Michael Ostap. Oxford: Academic Press, pp. 298-320 (2012). [LINK]
Dumont S. Chromosome segregation: Spindle mechanics come to life. Current Biology 21, R688-690 (2011). [PDF]
Dumont S, Mitchison TJ. Force and length in the mitotic spindle. Current Biology 19, R749-R761 (2009). [PDF]
Dumont S, Mitchison TJ. Compression regulates spindle length by a mechanochemical switch at the poles. Current Biology 19, 1086-1095 (2009). [PDF]
Commentaries: Nature, The Scientist
Wühr M, Dumont S, Groen AC, Needleman DJ, Mitchison TJ. How does a millimeter-sized cell find its center? Cell Cycle 8, 1115-1121 (2009). [PDF]
Wühr M, Chen Y, Dumont S, Groen AC, Needleman DJ, Salic A, Mitchison TJ. Evidence for an upper limit to mitotic spindle size. Current Biology 18, 1-6 (2008). [PDF]
Single-molecule biophysics:
*Equal contribution
Cheng W*, Dumont S*, Tinoco I Jr, Bustamante C. NS3 helicase actively separates RNA strands and senses sequence barriers ahead of the opening fork. PNAS 104, 13954-13959 (2007). [PDF]
Commentary: Science
Dumont S*, Cheng W*, Serebrov V, Beran RK, Tinoco I Jr, Pyle AM, Bustamante C. RNA unwinding mechanism of HCV NS3 helicase and its coordination by ATP. Nature 439, 105-108 (2006). [PDF]
Commentaries: NSMB, Hepatology, F1000
Onoa B*, Dumont S*, Liphardt J, Smith SB, Tinoco I Jr, Bustamante C. Identifying the kinetic barriers to mechanical unfolding of the T. thermophila ribozyme. Science 299, 1892-1895 (2003). [PDF]
Commentary: F1000
Liphardt J, Dumont S, Smith SB, Tinoco I Jr, Bustamante C. Equilibrium information from nonequilibrium measurements in an experimental test of Jarzynski's equality. Science 296, 1832-1835 (2002). [PDF]
Commentaries: Science